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tem:procedures
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Procedures
Start work
- HT→FEG Standby Mode→Normal (it takes ~13min)
- Mount sample
- Check nitrogen pressure!
- Insert holder till the first notch Air→Pump (wait for green light)
- Fill ACD with liquid nitrogen (in two steps)
- Fully insert holder
- Select holder type!
End work
- Stage Neutral
- Check nitrogen pressure!
- Pull holder till last notch
- Pump→Air, wait for nitrogen and remove the holder
- Mount ACD heater, Maintenance→ACD & Bake→ACD Heat→ON (turns off automatically)
- HT→FEG Standby Mode→Standby
Alignment
- GUNA(F3) Spot1 center with SHIFT ↔ BRIGHT TILT Spot5 center with SHIFT
- GUNA(F3) and Anode wobler(F5) make symetric with DEF
- COND STIG: make round with DEF
- STD FOCUS adjust Z (minimal contrast, no hallo, or no motion with IMAGE WOBB)
- Tilt and shift purity (at correct focus, and diffraction settings!)
- OBJ wobler (F4) make feature stationary with DEF(BRIGHT TILT)
- make fft round with DEF(OBJ STIG)
- SPECTRUM→move ZLP to center using FL lens
- Filter→Slit→IN move slit to center using position
- back to IMAGE, center circle with Isochromacity
- center diffraction with DEF(PLA)
- focus diffraction with DIFF FOCUS at Alpha 3, camera length 200cm, condensor fully CW
After bakeout
- remove mechanical safeties from EDX detector valve and beam valve
- Cycle BEAM VALVE with HT OFF
- Open EDX detector valve with BEAM VALVE closed
- Conditioning knob → COND
- HT→HT Conditioning→Auto Procedure→Start (it takes ~60 min)
- Conditioning knob → OPERATE
Beam shower
- Camera length 150cm
- CLA out, SAA #4, HCA #4
- CL1 0x8000, CL2 0x6000 (load STEMBeamShower.flc in FLC Panel)
- wait 10-15 min
After power failure
- reset chiller complaining about closed outlet
- (on-off HT breaker at the back of power supply unit)
- press (longer) “I” on the front of the microscope
- (Lenses on - front of the microscope)
- (EDX detector controller on-off, while SIP is not running (ACD on))
- watch vacuum
- (HT conditioning: switch knob to Cond, Auto procedure start, switch knob back to Operate)
- HT → Auto HT & Emission → Start up
Normal vacuum levels
| Column/PIG1 | ~25 uA | SIP starts at ? uA |
| Specimen/PIG4 | ~25 uA | ~250 uA without holder, pump LED goes green at 28 uA |
| Camera/PIG3 | ~25 uA | |
| RT1/PIG5 | ~80 uA | |
| RT2/PIG6 | ~25 uA | |
| 20l SIP | <1 uA | |
| 60l SIP | ~1 uA | |
| Column SIP | 200 uA | 800 uA with specimen without LN2 in ACD |
tem/procedures.txt · Last modified: 2021/10/25 08:53 (external edit)
