===== Procedures ===== ==== Start work ==== * HT->FEG Standby Mode->Normal (it takes ~13min) * Mount sample * Check nitrogen pressure! * Insert holder till the first notch Air->Pump (wait for green light) * Fill ACD with liquid nitrogen (in two steps) * Fully insert holder * Select holder type! ==== End work ==== * Stage Neutral * Check nitrogen pressure! * Pull holder till last notch * Pump->Air, wait for nitrogen and remove the holder * Mount ACD heater, Maintenance->ACD & Bake->ACD Heat->ON (turns off automatically) * HT->FEG Standby Mode->Standby ==== Alignment ==== * GUNA(F3) Spot1 center with SHIFT <-> BRIGHT TILT Spot5 center with SHIFT * GUNA(F3) and Anode wobler(F5) make symetric with DEF * COND STIG: make round with DEF * STD FOCUS adjust Z (minimal contrast, no hallo, or no motion with IMAGE WOBB) * Tilt and shift purity (at correct focus, and diffraction settings!) * OBJ wobler (F4) make feature stationary with DEF(BRIGHT TILT) * make fft round with DEF(OBJ STIG) * SPECTRUM->move ZLP to center using FL lens * Filter->Slit->IN move slit to center using position * back to IMAGE, center circle with Isochromacity * center diffraction with DEF(PLA) * focus diffraction with DIFF FOCUS at Alpha 3, camera length 200cm, condensor fully CW ==== After bakeout ==== * remove mechanical safeties from EDX detector valve and beam valve * Cycle BEAM VALVE with HT OFF * Open EDX detector valve with BEAM VALVE closed * Conditioning knob -> COND * HT->HT Conditioning->Auto Procedure->Start (it takes ~60 min) * Conditioning knob -> OPERATE ==== Beam shower ==== * Camera length 150cm * CLA out, SAA #4, HCA #4 * CL1 0x8000, CL2 0x6000 (load STEMBeamShower.flc in FLC Panel) * wait 10-15 min ==== After power failure ==== * reset chiller complaining about closed outlet * (on-off HT breaker at the back of power supply unit) * press (longer) "I" on the front of the microscope * (Lenses on - front of the microscope) * (EDX detector controller on-off, while SIP is not running (ACD on)) * watch vacuum * (HT conditioning: switch knob to Cond, Auto procedure start, switch knob back to Operate) * HT -> Auto HT & Emission -> Start up ==== Normal vacuum levels ==== | Column/PIG1 | ~25 uA | SIP starts at ? uA | | Specimen/PIG4 | ~25 uA | ~250 uA without holder, pump LED goes green at 28 uA | | Camera/PIG3 | ~25 uA | | | RT1/PIG5 | ~80 uA | | | RT2/PIG6| ~25 uA | | | 20l SIP | <1 uA | | 60l SIP | ~1 uA | | Column SIP | 200 uA | 800 uA with specimen without LN2 in ACD |